文献情報のみ A simplified purification method for AAV variant by polyethylene glycol aqueous two-phase partitioning Cell Pellet→Lysis→Nuclease→PEG8000-NaCl Precipitation. Replace from ultracentrifugation to precipitation procedure 10% PEG8000, 13.2% (HN4)2SO4 Culture Supernatant + PEG solution (1/4 vol)→stir slowly 4℃, 1hr→4℃, 3hrs without string to allow full peciptation 40% PEG 8000, 2.4% NaCl・・・
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